Anti-HLA-DP (MHC II)(HLA-DPB1/2862R)

Anti-HLA-DP (MHC II)(HLA-DPB1/2862R)

• Description: Recognizes a non-polymorphic determinant of DP-MHC class II. MHC class II antigens are transmembrane glycoproteins of non-covalently linked alpha (33-35 kDa) and beta (27-30 kDa) chains. It reportedly reacts with B- non-T, non-B cell lines but not with T- and myeloid cell lines and leukemias. Differential expression of MHC class II antigens on fetal and adult lymphocytes, malignant B cells appears to reflect the stage of cell differentiation which may be useful in the study of lymphoproliferative disorders. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: HLA class II histocompatibility antigen DP alpha 1 chain; HLA DP1A; HLA SB alpha chain; HLADP; HLADPA1; HLASB; HLASB histocompatibility type; Human MHC class II HLA SB alpha; Major histocompatibility complex class II DP alpha 1; MHC class II DP3 alpha; MHC class II DPA1; PLT1; Primed lymphocyte test 1
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: HLA-DPB1
• Gene ID: 3115
• NCBI Gene ID: 347270
• UniProt: P04440
• Cellular Locus: Plasma membrane
• Host: Rabbit
• Species Reactivity: Human
• Immunogen: Non-T, non-B human acute lymphoblastic leukemia REH6 cell line
• Target Antigen: HLA-DP
• Clonality: Recombinant Monoclonal
• Isotype: IgG
• Clone: HLA-DPB1/2862R
• Conjugation: Purified, BSA-free
• Source: Animal
• Applications: Flow (verified) | IF (verified)
• Validated Applications: FC, IF
• Positive Control: Raji cells. Tonsil or lymph node
• Concentration: 1 mg/mL
• Buffer: PBS, no BSA, no azide
• Molecular Weight: 33-35 kDa
• Additionnal Information: ELISA: For coating use Ab at 2-4 ug/mL, order Ab without BSA; Flow cytometry: 1-2 ug/million cells; Immunofluorescence: 1-2 ug/mL; Optimal dilution for a specific application should be determined.|Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody
• Shipping Conditions: Room temperature
• Storage Conditions: -35°C to -5°C ; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years