Anti-CD54 / ICAM-1(15.2)

Anti-CD54 / ICAM-1(15.2)

• Description: Recognizes an 85-115 kDa protein (variation with cell type), identified as intercellular adhesion molecule (ICAM-1). It has 7 potential N-linked glycosylation sites. ICAM-1 is a single chain glycoprotein of Ig supergene family, present on unstimulated endothelial cells (EC) and on a variety of other cell types including activated fibroblasts, EC, macrophages, and lymphocytes. ICAM-1 mediates cell adhesion by binding to integrins CD11a/CD18 (leukocyte adhesion molecule, LFA-1) and to CD11b/CD18 (Mac-1). This interaction enhances antigen-specific T-cell activation. ICAM-1 also binds to CD43 and to Plasmodium falciparum infected RBCs. ICAM-1 may also be related to progression and metastasis of tumors. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: CD54; Cell surface glycoprotein P3.58; Human rhinovirus receptor; ICAM-1; Intercellular adhesion molecule 1; Ly 47; Major group rhinovirus receptor; MALA2; MyD10; Surface antigen of activated B cells
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: ICAM1
• Gene ID: 3383
• NCBI Gene ID: 643447
• UniProt: P05362
• Cellular Locus: Plasma membrane
• Host: Mouse
• Species Reactivity: Human, Pig
• Immunogen: Human monocytes.
• Target Antigen: CD54 | ICAM-1
• Clonality: Monoclonal
• Isotype: IgG1 κ
• Clone: 15.2
• Conjugation: Purified, BSA-free
• Source: Animal
• Applications: Flow, surface (published) | Functional studies (published) | IF (published) | IHC, frozen (published)
• Validated Applications: FC, IF, IHC,
• Field of Research: Immunology
• Positive Control: Raji cells, MOLT 4, HeLa or SW480 cells. Human tonsil.
• Concentration: 1 mg/mL
• Buffer: PBS, no BSA, no azide
• Molecular Weight: 85-115 kDa
• References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. J Leukocyte Biol (1998) 64: 519. (Flow, surface) Inv Ophthalmol Visual Sci (2003) 44: 1888-1896. (IHC, frozen) Blood (1999) 93(11): 3723-3735. (IF) J Cell Biol (1992) 116(6): 1527-1535. (functional studies)
• Shipping Conditions: Room temperature
• Storage Conditions: -35°C to -5°C ; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years