Anti-CD54 / ICAM-1(15.2)

• Description:

  Recognizes an 85-115 kDa protein (variation with cell type), identified as intercellular adhesion molecule (ICAM-1). It has 7 potential N-linked glycosylation sites. ICAM-1 is a single chain glycoprotein of Ig supergene family, present on unstimulated endothelial cells (EC) and on a variety of other cell types including activated fibroblasts, EC, macrophages, and lymphocytes. ICAM-1 mediates cell adhesion by binding to integrins CD11a/CD18 (leukocyte adhesion molecule, LFA-1) and to CD11b/CD18 (Mac-1). This interaction enhances antigen-specific T-cell activation. ICAM-1 also binds to CD43 and to Plasmodium falciparum infected RBCs. ICAM-1 may also be related to progression and metastasis of tumors. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  CD54; Cell surface glycoprotein P3.58; Human rhinovirus receptor; ICAM-1; Intercellular adhesion molecule 1; Ly 47; Major group rhinovirus receptor; MALA2; MyD10; Surface antigen of activated B cells

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  ICAM1

• Gene ID:

  3383

• NCBI Gene ID:

  643447

• UniProt:

  P05362

• Cellular Locus:

  Plasma membrane

• Host:

  Mouse

• Species Reactivity:

  Human, Pig

• Immunogen:

  Human monocytes.

• Target Antigen:

  CD54 | ICAM-1

• Clonality:

  Monoclonal

• Isotype:

  IgG1 κ

• Clone:

  15.2

• Conjugation:

  Purified, with BSA

• Source:

  Animal

• Applications:

  Flow, surface (published) | Functional studies (published) | IF (published) | IHC, frozen (published)

• Validated Applications:

  FC, IF, IHC,

• Field of Research:

  Immunology

• Positive Control:

  Raji cells, MOLT 4, HeLa or SW480 cells. Human tonsil.

• Concentration:

  0.2 mg/mL

• Buffer:

  PBS, 0.05% BSA, 0.05% azide

• Molecular Weight:

  85-115 kDa

• References & Citations:

  Note: References for this clone sold by other suppliers may be listed for expected applications. J Leukocyte Biol (1998) 64: 519. (Flow, surface) Inv Ophthalmol Visual Sci (2003) 44: 1888-1896. (IHC, frozen) Blood (1999) 93(11): 3723-3735. (IF) J Cell Biol (1992) 116(6): 1527-1535. (functional studies)

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4