Anti-SIGLEC1 / CD169 / Sialoadhesin(HSn 7D2), Biotin conjugate

Anti-SIGLEC1 / CD169 / Sialoadhesin(HSn 7D2), Biotin conjugate

• Description: Two families of mammalian lectin-like adhesion molecules, the selectins and the sialoadhesins, bind glycoconjugate ligands in a sialic acid-dependent manner. The sialic acid-binding immunoglobulin superfamily lectins, designated siglecs or sialoadhesins, are immunoglobulin superfamily members that recognize sialylated ligands. The common sialic acids of mammalian cells are N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc). The human Siglec-1 gene maps to chromosome 20p13 and encodes a 1, 709 amino acid protein, also known as CD169. Alternative splicing of the Siglec-1 gene produces a variant, encoding a type I transmembrane protein isoform that is soluble rather than membrane-bound. Studies have shown human Siglec-1 has greater affinity for Neu5Ac over Neu5Gc. Siglec-1 is a sialic acid-binding receptor that is expressed in hemopoietic cells. It mediates local cell-cell interactions in lymphoid tissues and can be detected at contact points of macrophages with other macrophages, sinus-lining cells and reticulum cells. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: CD169; CD169 antigen; Sialic acid binding immunoglobulin like lectin 1; Sialic acid-binding Ig-like lectin 1; Sialoadhesin; Siglec-1; SN
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: SIGLEC1
• Gene ID: 6614
• NCBI Gene ID: 31869
• UniProt: Q9ZZ2
• Cellular Locus: Plasma membrane|Secreted (extracellular)
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Fc fusion protein containing N-terminal 4 domains of human sialoadhesin.
• Target Antigen: CD169 | Sialoadhesin | SIGLEC1
• Clonality: Monoclonal
• Isotype: IgG1 κ
• Clone: HSn 7D2
• Conjugation: Biotin
• Source: Animal
• Applications: Flow, surface (published) | Functional studies (published) | IHC, FFPE (published) | WB (published)
• Validated Applications: FC, IHC, FFPE, WB
• Positive Control: Human spleen, human peripheral blood monocytes or MCF7 cell.
• Concentration: 0.1 mg/mL
• Buffer: PBS, 0.1% BSA, 0.05% azide
• Molecular Weight: 185 kDa
• References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. Cancer Sci (2013) 104(9): 1237-1244. (WB; cell ELISA; IHC, FFPE) Rheumatology (2014) 53(2): 250-259. (Flow, surface; functional studies)
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years