Anti-HER-4 / ERBB4(ERBB4/2581), CF594 conjugate

Anti-HER-4 / ERBB4(ERBB4/2581), CF594 conjugate

• Description: The EGF receptor family comprises several related receptor tyrosine kinases that are frequently overexpressed in a variety of carcinomas. Members of this receptor family include EGFR (HER1), Neu (ErbB-2, HER2), ErbB-3 (HER3) and ErbB-4 (HER4), which form either homodimers or heterodimers upon ligand binding. The gene encoding ErbB-4 is expressed as a full-length protein, which produces a short membrane-anchored cytoplasmic domain fragment and a long ectodomain fragment. The short fragment is heavily tyrosine phosphorylated and possesses tyrosine kinase catalytic activity toward an exogenous substrate. Proteolytic cleavage of ErbB-4 is promoted by the binding of heregulin. ErbB-4 is involved in cell proliferation and differentiation and its expression is highest in breast carcinoma cell lines, normal skeletal muscle, heart, pituitary, brain and cerebellum. Its expression in breast cancer, pediatric brain cancer and other types of carcinomas has been reported in studies which suggest ErbB4 expression is involved in both normal tissue development and carcinogenesis. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: Avian erythroblastic leukemia viral oncogene homolog 4; HER4; human epidermal growth factor receptor 4; p180erbB4; Proto-oncogene-like protein c-ErbB-4
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: ERBB4
• Gene ID: 2066
• NCBI Gene ID: 390729
• UniProt: Q15303
• Cellular Locus: Plasma membrane|Nucleus & cytoplasm
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Recombinant fragment (around aa 1116-1269) of human ERBB4 (HER4) protein (exact sequence is proprietary)
• Target Antigen: ERBB4 | HER-4
• Clonality: Monoclonal
• Isotype: IgG1 κ
• Clone: ERBB4/2581
• Conjugation: CF594
• Disease: Tumor
• Source: Animal
• Applications: IHC, FFPE (verified)
• Validated Applications: IHC, FFPE
• Positive Control: Human breast, brain or kidney tissues (IHC).
• Concentration: 0.1 mg/mL
• Buffer: PBS, 0.1% BSA, 0.05% azide
• Molecular Weight: 180 kDa (precursor); 80/120 kDa (cleaved)
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years