Anti-CD40 / TNFRSF5 / CD40L-Receptor (C40/2383)

• Description:

  CD40 is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. CD40 is expressed on B-lymphocytes, follicular dendritic cells, bone marrow-derived dendritic cells, thymic epithelium, and interdigitating cells in the T-cell zones of secondary lymphoid organs.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  B-cell surface antigen CD40; Bp50; CD40; CD40L receptor; GP39; HIGM1; IGM; IMD3; p50; TBAM; TNF receptor superfamily member 5; TNFRSF5; TRAP

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  CD40

• Gene ID:

  958

• NCBI Gene ID:

  472860

• UniProt:

  P25942

• Cellular Locus:

  Plasma membrane|Secreted (extracellular)

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Recombinant fragment of human CD40 protein (around aa 29-107) (exact sequence is proprietary)

• Target Antigen:

  CD40 | CD40L-Receptor | TNFRSF5

• Clonality:

  Monoclonal

• Isotype:

  IgG2b κ

• Clone:

  C40/2383

• Conjugation:

  Purified, BSA-free

• Source:

  Animal

• Applications:

  Flow (verified) | IHC, FFPE (verified)

• Validated Applications:

  FC, IHC, FFPE

• Field of Research:

  Immunology

• Positive Control:

  L-428 Cells. Tonsil, Spleen or Thymus

• Concentration:

  1 mg/mL

• Buffer:

  PBS, no BSA, no azide

• Molecular Weight:

  43 kDa

• Additionnal Information:

  Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunohistology (formalin): 1-2 ug/mL for 30 minutes at RT|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes|Optimal dilution for a specific application should be determined by user

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  -35°C to -5°C ; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4