Anti-Occludin (Marker of Early Blood Brain Barrier Damage)(OCLN/2181), CF647 conjugate

• Description:

  Occludin is a tetraspan integral membrane protein in epithelial and endothelial tight junction (TJ) structures that can contain two extracellular loops. The protein exists in a variety of phosphorylated forms. Phosphorylation is involved in regulating both the localization and the function of Occludin. Expression of Occludin is upregulated by polyunsaturated fatty acids, increasing trans-endothelial cell resistance and reducing cellular permeability to large molecules. The level of Occludin varies greatly depending on tissue; in brain tissue, Occludin is highly expressed at cell-cell contact sites. Non-neural tissues show lower expression and discontinuous distribution. Occludin is a tight junction protein that is a key structural component of the blood-brain barrier (BBB). Degradation of Occludin is frequently seen in ischemic stroke and contributes to BBB disruption. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  BLCPMG; Occludin; OCLN; Phosphatase 1 regulatory subunit 115; PTORCH1; Tight junction protein occludin

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  OCLN

• Gene ID:

  100506658

• NCBI Gene ID:

  592605

• UniProt:

  Q16625

• Cellular Locus:

  Cell junctions|Plasma membrane

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Recombinant fragment of human Occludin protein (around aa 282-415) (exact sequence is proprietary)

• Target Antigen:

  Occludin

• Clonality:

  Monoclonal

• Isotype:

  IgG1 κ

• Clone:

  OCLN/2181

• Conjugation:

  CF647

• Source:

  Animal

• Applications:

  IHC, FFPE (verified) | WB (verified)

• Validated Applications:

  IHC, FFPE, WB

• Positive Control:

  HepG2 cells. Kidney.

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  60-82 kDa

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4