Anti-Tyrosinase (Melanoma Marker) (TYR/2024R)

• Description:

  This antibody recognizes a cluster of proteins between 70-80 kDa, identified as tyrosinase. Occasionally a minor band at 55 kDa is also detected. This MAb shows no cross-reaction with MAGE-1 and tyrosinase-related protein 1, TRP-1/gp75. Tyrosinase is a copper-containing metalloglycoprotein that catalyzes several steps in the melanin pigment biosynthetic pathway; the hydroxylation of tyrosine to L-3,4-dihydroxy-phenylalanine (dopa), and the subsequent oxidation of dopa to dopaquinone. Mutations of the tyrosinase gene occur in various forms of albinism. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Staining of melanomas with this MAb shows tyrosinase in melanotic as well as amelanotic variants. This MAb is a useful marker for melanocytes and melanomas.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  ATN, CMM8, LB24-AB, Monophenol monooxygenase, OCA1, OCA1A, Oculocutaneous albinism IA, SHEP3, SK29-AB, Tumor rejection antigen AB, TYR

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  TYR

• Gene ID:

  7299

• NCBI Gene ID:

  503555

• UniProt:

  P14679

• Cellular Locus:

  Vesicular

• Host:

  Rabbit

• Species Reactivity:

  Human

• Immunogen:

  Recombinant full-length human tyrosinase (TYR) protein

• Target Antigen:

  Tyrosinase

• Clonality:

  Recombinant Monoclonal

• Isotype:

  IgG

• Clone:

  TYR/2024R

• Conjugation:

  Purified, BSA-free

• Disease:

  Tumor

• Source:

  Animal

• Applications:

  IHC, FFPE (verified)

• Validated Applications:

  IHC, FFPE

• Field of Research:

  Cancer

• Positive Control:

  SK-MEL-19, SK-MEL-30 cells or Melanoma.

• Concentration:

  1 mg/mL

• Buffer:

  PBS, no BSA, no azide

• Molecular Weight:

  70-80 kDa

• Additionnal Information:

  Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunohistology (formalin): 0.5-1 ug/mL for 30 minutes at RT|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA pH 9.0 or 10 mM citrate buffer pH6.0 for 10-20 minutes followed by cooling at RT for 20 minutes|Optimal dilution for a specific application should be determined by user

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  -35°C to -5°C ; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4