Anti-VEGF (Vascular Endothelial Growth Factor)(VG76e), Biotin conjugate

Anti-VEGF (Vascular Endothelial Growth Factor)(VG76e), Biotin conjugate

• Description: This MAb recognizes proteins of 19-22 kDa (reducing) and 38 kDa-44 kDa (non-reducing), identified as various isoforms of Vascular Endothelial Growth Factor or Vascular Permeability Factor (VEGF/VPF). It is highly specific to VEGF, which is a homodimeric, disulfide-linked glycoprotein with a close homology to platelet-derived growth factor (PDGF). There are multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues. The smaller two isoforms, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression and metastasis. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: MVCD1; Vascular Endothelial Growth Factor A (VEGF-A); Vascular Permeability Factor (VPF); VEGFA
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: VEGFA
• Gene ID: 7422
• NCBI Gene ID: 73793
• UniProt: P15692
• Cellular Locus: Plasma membrane|Secreted (extracellular)
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Human VEGF189 recombinant protein
• Target Antigen: VEGF
• Clonality: Monoclonal
• Isotype: IgG1 κ
• Clone: VG76e
• Conjugation: Biotin
• Source: Animal
• Applications: Functional studies (published) | IHC, FFPE (published) | WB (published)
• Validated Applications: IHC, FFPE, WB
• Positive Control: Tumor cells in astrocytomas, breast or ovarian carcinomas.
• Concentration: 0.1 mg/mL
• Buffer: PBS, 0.1% BSA, 0.05% azide
• Molecular Weight: 19-22 kDa (reducing) and 38 kDa-44 kDa (non-reducing)
• Additionnal Information: ELISA: For coating, order Ab without BSA; Immunofluorescence: 1-2 ug/mL; Optimal dilution for a specific application should be determined.|Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody
• References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. Endocrinology (2000) 141(3): 995-1000. (WB, functional studies) J Vet Diag Invest (2010) 22(1):105-108. (IHC, FFPE)
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years