Anti-CD4 (T-Helper/Inducer Cell Marker)(RIV7)

Anti-CD4 (T-Helper/Inducer Cell Marker)(RIV7)

• Description: Recognizes a protein of 55 kDa, identified as CD4. It is a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigens and is also a receptor for the human immunodeficiency virus. This protein is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified._x000D_ _x000D_ Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_
• Synonyms: L3T4; Leu3; Ly-4; Lymphocyte antigen CD4; p55; T cell antigen T4/LEU3; T cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: CD4
• Gene ID: 920
• NCBI Gene ID: 631659
• UniProt: P01730
• Cellular Locus: Plasma membrane
• Host: Mouse
• Species Reactivity: Human, Monkey
• Immunogen: Human peripheral lymphocytes
• Target Antigen: CD4
• Clonality: Monoclonal
• Isotype: IgG2a κ
• Clone: RIV7
• Conjugation: Purified, with BSA
• Disease: Tumor
• Source: Animal
• Applications: Functional studies (published) | Flow, surface (verified) | IF (verified)
• Validated Applications: FC, IF
• Positive Control: Human peripheral blood mononuclear cells (PBMCs), CCRF-CEM or HL-60. Lymph node or tonsil.
• Concentration: 0.2 mg/mL
• Buffer: PBS, 0.05% BSA, 0.05% azide
• Molecular Weight: 55 kDa
• Additionnal Information: Functional studies: Order Ab without azide; Flow cytometry: 1-2 ug/million cells in 0.1 mL; Immunofluorescence: 1-2 ug/mL; Optimal dilution for a specific application should be determined.
• References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. Knapp W. et al. (eds) Leukocyte Typing IV, p314-330, Oxford University Press, Oxford, 1989. Br J Haematol (2004) 128: 73-81. (functional studies)
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years