Anti-CD4 (T-Helper/Inducer Cell Marker)(RIV7)

• Description:

  Recognizes a protein of 55 kDa, identified as CD4. It is a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigens and is also a receptor for the human immunodeficiency virus. This protein is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified._x000D_ _x000D_ Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_

• Synonyms:

  L3T4; Leu3; Ly-4; Lymphocyte antigen CD4; p55; T cell antigen T4/LEU3; T cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4

• CAS Number:

  9007-83-4

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  CD4

• Gene ID:

  920

• NCBI Gene ID:

  631659

• UniProt:

  P01730

• Cellular Locus:

  Plasma membrane

• Host:

  Mouse

• Species Reactivity:

  Human, Monkey

• Immunogen:

  Human peripheral lymphocytes

• Target Antigen:

  CD4

• Clonality:

  Monoclonal

• Isotype:

  IgG2a κ

• Clone:

  RIV7

• Conjugation:

  Purified, with BSA

• Disease:

  Tumor

• Source:

  Animal

• Applications:

  Functional studies (published) | Flow, surface (verified) | IF (verified)

• Validated Applications:

  FC, IF

• Positive Control:

  Human peripheral blood mononuclear cells (PBMCs), CCRF-CEM or HL-60. Lymph node or tonsil.

• Concentration:

  0.2 mg/mL

• Buffer:

  PBS, 0.05% BSA, 0.05% azide

• Molecular Weight:

  55 kDa

• Additionnal Information:

  Functional studies: Order Ab without azide; Flow cytometry: 1-2 ug/million cells in 0.1 mL; Immunofluorescence: 1-2 ug/mL; Optimal dilution for a specific application should be determined.

• References & Citations:

  Note: References for this clone sold by other suppliers may be listed for expected applications. Knapp W. et al. (eds) Leukocyte Typing IV, p314-330, Oxford University Press, Oxford, 1989. Br J Haematol (2004) 128: 73-81. (functional studies)

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years