Recombinant chimeric Dpo4 [synthetic construct]

Recombinant chimeric Dpo4 [synthetic construct]

• CAS Number: 9000-83-3
• UniProt: ABA03150.1
• Expression Region: 1-353aa
• Organism: synthetic construct
• Target Sequence: MIVLFVDFDYFFAQVEEVLNPELKGKPVAVCVFSGRFKDSGAIATANYEARKLGIKSGMPIPKAKEIAPNAIYLPIRKDLYKQVSDRIMYGILSKYSSKIEIASIDEAYLDITDRVKDYYEAYQLGKKIKDEIYQKEKITVTIGIAPNKVFAKIIAEMNKPNGLGILKPEEVEGFIRSLPIEEVPGVGDSIYSKLKEMEIKYLYDVLKVDFEKLKKEIGKSKASYLYSLARDEYNEPIRTRVRKSIGRIVTMKRNSRNLEEIKPYLFRAIEESYYKLDKRIPKAIHVVAVTEDLDIVSRGRTFPHGISKETAYSESVKLLQKILEEDERKIRRIGVRFSKFIEAIGLDKFFDT
• Tag: N-terminal 6xHis-tagged
• Source: E.coli
• Field of Research: others
• Assay Type: In Stock Protein
• Purity: Greater than 90% as determined by SDS-PAGE.
• Activity: Not Test
• Length: Full Length
• Form: Liquid or Lyophilized powder
• Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Molecular Weight: 44.7 kDa
• References & Citations: McDonald JP, Hall A, Gasparutto D, Cadet J, Ballantyne J, Woodgate R. Novel thermostable Y-family polymerases: applications for the PCR amplification of damaged or ancient DNAs. Nucleic Acids Res. 2006 Feb 18;34 (4):1102-11. doi: 10.1093/nar/gkj512. PMID: 16488882; PMCID: PMC1373694.
• Storage Conditions: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.