Recombinant chimeric Dpo4 [synthetic construct]

• CAS Number:

  9000-83-3

• UniProt:

  ABA03150.1

• Expression Region:

  1-353aa

• Organism:

  synthetic construct

• Target Sequence:

  MIVLFVDFDYFFAQVEEVLNPELKGKPVAVCVFSGRFKDSGAIATANYEARKLGIKSGMPIPKAKEIAPNAIYLPIRKDLYKQVSDRIMYGILSKYSSKIEIASIDEAYLDITDRVKDYYEAYQLGKKIKDEIYQKEKITVTIGIAPNKVFAKIIAEMNKPNGLGILKPEEVEGFIRSLPIEEVPGVGDSIYSKLKEMEIKYLYDVLKVDFEKLKKEIGKSKASYLYSLARDEYNEPIRTRVRKSIGRIVTMKRNSRNLEEIKPYLFRAIEESYYKLDKRIPKAIHVVAVTEDLDIVSRGRTFPHGISKETAYSESVKLLQKILEEDERKIRRIGVRFSKFIEAIGLDKFFDT

• Tag:

  N-terminal 6xHis-tagged

• Source:

  E.coli

• Field of Research:

  others

• Assay Type:

  In Stock Protein

• Purity:

  Greater than 90% as determined by SDS-PAGE.

• Activity:

  Not Test

• Length:

  Full Length

• Form:

  Liquid or Lyophilized powder

• Buffer:

  If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Molecular Weight:

  44.7 kDa

• References & Citations:

  McDonald JP, Hall A, Gasparutto D, Cadet J, Ballantyne J, Woodgate R. Novel thermostable Y-family polymerases: applications for the PCR amplification of damaged or ancient DNAs. Nucleic Acids Res. 2006 Feb 18;34 (4):1102-11. doi: 10.1093/nar/gkj512. PMID: 16488882; PMCID: PMC1373694.

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.