Rabbit anti Sheep erythrocyte

Rabbit anti Sheep erythrocyte

• Background: To sensitize cells in hemolytic complement assays and for lymphocytic screening procedures.
• CAS Number: 9007-83-4
• Host: Rabbit
• Clone: Polyclonal
• Source: Washed whole erythrocytes were prepared from fresh blood and used for intravenous immunization. Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Assay Principle: Precipitation assays. Hemolytic titre at which 50% lysis occurs following mixing of equal volumes of antiserum, guinea pig complement (at a dilution of 1:60) and 10 8 RBC/mL not less than 1:3,000 when tested with sheep erythrocytes.
• Form: Delipidated, heat inactivated, lyophilized, stable whole antiserum; No preservative added; Total protein and IgG concentrations in the antiserum are comparable to those of pooled; Normal rabbit serum; No foreign proteins added; Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water
• Precautions: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• Storage Conditions: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C; Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2); Repeated thawing and freezing should be avoided; If a slight precipitation occurs upon storage, this should be removed by centrifugation; It will not affect the performance of the antiserum Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day