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NC2 alpha Antibody / DRAP1

CAT: 0013-GTR18318379-01Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18318379-01Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
NC2 (negative cofactor 2) is a dimeric histone-fold complex that represses RNA polymerase II transcription through binding to TBP and inhibiting the transcription factors TFIIA and TFIIB. NC2 consists of two subunits, termed NC2a and NC2beta, and these subunits dimerize and bind to TBP-promoter complexes via histone fold domains of the H2A-H2B type. NC2 associates with promoters in a manner that correlates with transcriptional activity and with occupancy by basal transcription factors. NC2 binds directly to DNA, and the binding of NC2 to TBP-promoter complexes affects the conformation of DNA, and results in the inhibition of TFIIB.
UniProt
Q14919
Reactivity
Human
Immunogen
Recombinant human protein was used as the immunogen for the NC2 alpha antibody.
Clonality
Monoclonal
Clone
PCRP-DRAP1-1A12
Conjugation
Unconjugated
Purification
Protein A/G affinity
Dilution
Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2ug/ml, Western blot: 2-4ug/ml
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the NC2 alpha antibody should be determined by the researcher.
Tested Applications
FACS, IF, WB
Host or Source
Mouse
Preservative
0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin, 0.05% sodium azide
Isotype
Mouse IgG2b, kappa

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