Rabbit anti-ERK1/2 Antibody

• Background:

  Rabbit polyclonal antibody to ERK1/2

• Synonyms:

  MAPK3; ERK1; PRKM3; Mitogen-activated protein kinase 3; MAP kinase 3; MAPK 3; ERT2; Extracellular signal-regulated kinase 1; ERK-1; Insulin-stimulated MAP2 kinase; MAP kinase isoform p44; p44-MAPK; Microtubule-associated protein 2 kinase; p44-ERK1; MAPK1; ERK2; PRKM1; PRKM2; Mitogen-activated protein kinase 1; MAP kinase 1; MAPK 1; ERT1; Extracellular signal-regulated kinase 2; ERK-2; MAP kinase isoform p42; p42-MAPK; Mitogen-activated protein kinase 2; MAP kinase 2; MAPK 2

• Host:

  Rabbit

• Reactivity:

  H, M, R, B, D, P

• Immunogen:

  KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human ERK1/2. The exact sequence is proprietary.

• Clonality:

  Polyclonal

• Applications:

  WB, IHC

• Purification Method:

  The antibody was purified by immunogen affinity chromatography.

• Concentration:

  1mg/mL

• Dilution:

  WB (1/500 - 1/1000), IH (1/100 - 1/200)

• Buffer:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

• Storage Conditions:

  Store at -20°C. Avoid repeated freeze / thaw cycles.

• WB Description:

  Western blot analysis of ERK1/2 expression in HeLa (A), NIH3T3 (B), PC12 (C) whole cell lysates.

• IHC description:

  Immunohistochemical analysis of ERK1/2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• Gene ID (Human):

  5595

• Gene ID (Mouse):

  26417; 26413

• Gene ID (Rat):

  50689; 116590

• SwissProt (Human):

  P27361; P28482

• SwissProt (Mouse):

  Q63844; P63085

• SwissProt (Rat):

  P21708; P63086

• CAS Number:

  9007-83-4