Rabbit anti-ERK1 Antibody

• Background:

  Rabbit polyclonal antibody to ERK1

• Synonyms:

  ERK1; PRKM3; Mitogen-activated protein kinase 3; MAP kinase 3; MAPK 3; ERT2; Extracellular signal-regulated kinase 1; ERK-1; Insulin-stimulated MAP2 kinase; MAP kinase isoform p44; p44-MAPK; Microtubule-associated protein 2 kinase; p44-ERK1

• Host:

  Rabbit

• Reactivity:

  H, M, R, P

• Immunogen:

  KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ERK1. The exact sequence is proprietary.

• Clonality:

  Polyclonal

• Applications:

  WB, IF/IC

• Purification Method:

  The antibody was purified by immunogen affinity chromatography.

• Concentration:

  1mg/mL

• Dilution:

  WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)

• Buffer:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

• Storage Conditions:

  Store at -20°C. Avoid repeated freeze / thaw cycles.

• WB Description:

  Western blot analysis of ERK1 expression in A431 (A), NIH3T3 (B), PC12 (C) whole cell lysates.

• IF/ICC description:

  Immunofluorescent analysis of ERK1 staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue) .

• Gene ID (Human):

  5595

• Gene ID (Mouse):

  26417

• Gene ID (Rat):

  50689

• SwissProt (Human):

  P27361

• SwissProt (Mouse):

  Q63844

• SwissProt (Rat):

  P21708

• CAS Number:

  9007-83-4