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Vimentin Rabbit Polyclonal Antibody

CAT: 0013-GTR18307730Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0013-GTR18307730Size:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Vimentin antibody detects vimentin, a type III intermediate filament protein with a predicted molecular weight of 54 kDa. Vimentin is highly expressed in cells of mesodermal origin as well as in some ectodermal cells, and is often used as a marker of mesenchymal stem cells. It is also utilized in combination with E-cadherin to study the morphological and phenotypic changes in cells undergoing epithelial–mesenchymal transition during development and tumor metastasis. In addition, vimentin antibody is a valuable reagent to stain neuronal precursors and developing radial glia cells.
Product Name Alternative
Vimentin
UniProt
P08670
Reactivity
Canine, Feline, Human, Mouse, Rat
Immunogen
Recombinant protein encompassing a sequence within the center region of human Vimentin. The exact sequence is proprietary.
Target
Vimentin
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Musculoskeletal & Connective Tissue Research
Purification
Purified by antigen-affinity chromatography.
Concentration
0.15 mg/ml (Please refer to the vial label for the specific concentration.)
Dilution
WB: 1:5000-1:50000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Form
Liquid
Molecular Weight
54
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive control: 293T
Tested Applications
ELISA, ICC, IHC, IHC-Fr, IHC-P, IP, WB
Host or Source
Rabbit
Preservative
PBS, 1% rAlbumin, 20% Glycerol, 0.025% ProClin 300.
Isotype
IgG

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