FAK (Phospho Ser722) rabbit pAb

FAK (Phospho Ser722) rabbit pAb

• Description: Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. Domain: The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions. Domain: The first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL. function: Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity. PTM: Phosphorylated on 6 tyrosine residues upon activation. similarity: Belongs to the protein kinase superfamily. Tyr protein kinase family. similarity: Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily. similarity: Contains 1 FERM domain. similarity: Contains 1 protein kinase domain. subcellular location: Constituent of focal adhesions. subunit: Interacts with CAS family members and with GIT1, SORBS1 and BCAR3. Interacts with RGNEF and SHB (By similarity). Interacts with TGFB1I1. tissue specificity: Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.
• Synonyms: Focal adhesion kinase 1 (FADK 1;EC 2.7.10.2;Focal adhesion kinase-related nonkinase;FRNK;Protein phosphatase 1 regulatory subunit 71;PPP1R71;Protein-tyrosine kinase 2;p125FAK;pp125FAK)
• Gene ID: 5747
• UniProt: Q05397
• Cellular Locus: Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cell cortex. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Nucleus. Cytoplasm, cytoskeleton, cilium basal body. Constituent of focal adhesions. Detected at microtubules.
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Synthesized peptide derived from human FAK (Phospho Ser722)
• Clonality: Polyclonal
• Validated Applications: WB, ELISA
• Stability: 1 year
• Concentration: 1 mg/mL
• Dilution: WB 1:1000-2000 ELISA 1:5000-20000
• Molecular Weight: 125kD
• Storage Conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.