PRK1 (phospho-Thr774)/PRK2 (phospho-Thr816) rabbit pAb

PRK1 (phospho-Thr774)/PRK2 (phospho-Thr816) rabbit pAb

• Description: Protein kinase N1 (PKN1) Homo sapiens The protein encoded by this gene belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008],
• Synonyms: Serine/threonine-protein kinase N1 (EC 2.7.11.13) (Protease-activated kinase 1) (PAK-1) (Protein kinase C-like 1) (Protein kinase C-like PKN) (Protein kinase PKN-alpha) (Protein-kinase C-related kinase 1) (Serine-threonine protein kinase N)
• Gene ID: 5585
• UniProt: Q16512
• Cellular Locus: Cytoplasm. Nucleus. Endosome. Cell membrane ; Peripheral membrane protein. Cleavage furrow. Midbody. Associates with chromatin in a ligand-dependent manner. Localization to endosomes is mediated via its interaction with RHOB. Association to the cell membrane is dependent on Ser-377 phosphorylation. Accumulates during telophase at the cleavage furrow and finally concentrates around the midbody in cytokinesis..
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Synthesized phosho peptide around human PRK1 (Thr774) and PRK2 (Thr816)
• Clonality: Polyclonal
• Validated Applications: WB, ELISA, IHC
• Stability: 1 year
• Concentration: 1 mg/mL
• Dilution: WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
• Molecular Weight: 103kD
• Storage Conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.