Anti-Hu CD100 FITC

Anti-Hu CD100 FITC

• Background: CD100, also known as semaphorin 4D, is a homodimerizing type I transmembrane glycoprotein containing an extracellular semaphorin domain. It is expressed on most hematopoietic cells with the exception of immature bone marrow cells, erythrocytes and platelets. A 120 kDa soluble form is generated from the transmembrane form by proteolytic cascade following primary T and B cell activation. It seems CD100 acts through dampening CD72-mediated negative signaling. CD100 promotes angiogenesis, invasive growth, proliferation and anti-apoptosis of cancer cells in vitro. Higher expression levels of CD100 correlate with poor survival in soft tissue sarcoma patients.
• Specifications: The mouse monoclonal antibody 133-1C6 recognizes an extracellular epitope of CD100, an approximately 150 kDa (when reduced) semaphorin family member expressed mainly on lymphocytes, NK cells, monocytes/macrophages and granulocytes, but also on some non-hematopoietic cells.
• Certification: RUO
• Host: Mouse
• Species Reactivity: Human
• Immunogen: PHA stimulated human PBL
• Target Antigen: CD100
• Isotype: IgM
• Clone: 133-1C6
• Applications: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 mL) is sufficient for 100 tests.
• Validated Applications: FC
• Format: FITC
• Buffer: Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
• References & Citations: *Jiang X, Björkström NK, Melum E: Intact CD100-CD72 interaction necessary for TCR-induced T cell proliferation. Front Immunol. 2017 Jun 308:765. doi: 10.3389/fimmu.2017.00765., URL: https://www.ncbi.nlm.nih.gov/pubmed/?term=intact+cd100-cd72+interaction,
• Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.