Rabbit anti-native Green Fluorescent Protein (Ammonium Sulfate fraction)

Rabbit anti-native Green Fluorescent Protein (Ammonium Sulfate fraction)

• Background: Green fluorescent protein (GFP) is a 27kD protein which was originally identified in the photo organs of Aequorea victoria (A. victoria) jellyfish. GFP is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. In A.victoria, calcium ions bind and activate the protein aequorin causing the release of blue fluorescence, which is then absorbed by GFP resulting in the release of green fluorescence. In the laboratory, the GFP protein has been used extensively as a reporter molecule to label, and study, cellular and subcellular proteins in living cells using a wide range of biological applications, including oncology, cardiovascular diseases, brain research and embryology, just to name a few. For the quantitation of the expression of a specific protein, tagged with GFP in these model systems, antibodies to GFP have proven to be of value in immunoblotting studies and ELISA protocols.
• UniProt: P42212 (A.victoria)
• Host: Rabbit
• Clone: Polyclonal
• Type: Primary Antibodies
• Source: The anti-GFP antibody is a rabbit polyclonal antiserum obtained from rabbits immunised with highly purified native GFP derived from Aequorea victoria jellyfish.
• Applications: WB, ELISA
• Purification Method: Ammonium sulfate precipitation
• Assay Principle: Western Blotting|ELISA
• Concentration: 1 mg/mL
• Form: Each vial contains 100 µL 1 mg/mL polyclonal antiserum in 10 mM TRIS buffer pH 7,2, containing 0,02% sodium azide The antibodies were purified by ammonium sulfate precipitation.
• Precautions: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• References & Citations: 1. Chalfie M, Tu Y, Euskirchen G, Ward WW, Prasher DC. (1994) Green fluorescent protein as a marker for gene expression. Science 263: 802-805. 2. UniProt: P42212 (A.victoria)
• Shipping Conditions: Ambient Temperature
• Storage Conditions: The antibody may be stored at +4°C; For prolonged storage prepare appropriate aliquots and store at or below -20°C; Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2); Repeated thawing and freezing should be avoided; Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day; If a slight precipitation occurs upon storage, this should be removed by centrifugation; It will not affect the performance or the concentration of the product