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IL1B Antibody (Biotin)

CAT: 0013-GTR18289418Size: 0.025 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18289418Size:0.025 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
IL1B Antibody (Biotin)
Product Name Alternative
Catabolin, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF)
UniProt
P01584
Reactivity
Human
Immunogen
Produced from sera of Goats pre-immunized with highly pure (>98%) recombinant hIL-1 beta. Anti-HumanIL-1beta specific antibody was purified by affinity chromatography and then biotinylated.
Target
IL1B
Clonality
Polyclonal
Conjugation
Biotin
Field of Research
Immunology & Inflammation
Purification
IL-1 beta specific antibody was purified by affinity chromatography and then biotinylated
Concentration
Batch dependent
Form
Lyophilized
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Sandwich ELISA: To detect hIL-1 beta by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Biorbyt’s Polyclonal Anti-Human IL-1 beta as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hIL-1 beta. Western Blot To detect hIL-1 beta by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-1 beta is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Tested Applications
ELISA, WB
NCBI Accession Number
P01584
Host or Source
Goat

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