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THPO Antibody (Biotin)

CAT: 0013-GTR18289406Size: 0.025 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18289406Size:0.025 mg
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Description
THPO Antibody (Biotin)
Product Name Alternative
Thrombopoietin, Megakaryocyte colony-stimulating factor, c-MPL Ligand, MGDF
UniProt
P40225
Reactivity
Human
Immunogen
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hTPO. Anti-Human TPO specific antibody was purified by affinity chromatography and then biotinylated.
Target
THPO
Clonality
Polyclonal
Conjugation
Biotin
Field of Research
Cell Biology
Purification
TPO specific antibody was purified by affinity chromatography and then biotinylated
Concentration
Batch dependent
Form
Lyophilized
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Sandwich ELISA: To detect hTPO by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Biorbyt’s Polyclonal Anti-Human TPO as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hTPO. Western Blot To detect hTPO by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTPO is 1.5 - 3.0 ng/lane, under either reducing or non-reducing condition
Tested Applications
ELISA, WB
NCBI Accession Number
P40225
Host or Source
Rabbit

UniProtKB · P40225

Thrombopoietin

TPO_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P40225
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
THPO
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
A1L3Y0, B7ZLR8, B9EGA8, Q13020, Q15790, Q15791, Q15792
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Molecular function

CytokineHormone

Disease

Disease variant

PTM

Disulfide bondGlycoprotein

Cellular component

Secreted

Domain

Signal

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