Anti-Ankyrin-G Antibody

Anti-Ankyrin-G Antibody

• Description: Mouse Monoclonal Antibody specific to Ankyrin-G
• Product Name Alternative: ANK-3, Ankyrin-G
• CAS Number: 9007-83-4
• Gene Name: ANK3
• NCBI Gene ID: ANK3
• UniProt: Q12955
• Cellular Locus: Cytoplasm, cytoskeleton, Cell projection, axon, Cell membrane, sarcolemma, Cell junction, synapse, postsynaptic cell membrane, Lysosome, Cell membrane, sarcolemma, T-tubule
• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Immunogen: Fusion protein corresponding to ~1,000 C-terminal amino acids of human Ankyrin G (accession no. NP_066267.2).
• Target Antigen: Ankyrin-3
• Target: Ankyrin-G
• Clonality: Monoclonal
• Isotype: IgG1
• Type: Antibody
• Applications: WB, ICC/IF
• Field of Research: Neuroscience
• Purification Method: Purified by Protein G affinity chromatography
• Concentration: 1.0 mg/mL
• Dilution: Dilute in PBS or medium that is identical to that used in the assay system.
• Format: Purified
• Form: Liquid
• Buffer: Phosphate Buffered Saline
• Function: In skeletal muscle, required for costamere localization of DMD and betaDAG1 (By similarity). Membrane-cytoskeleton linker. May participate in the maintenance/targeting of ion channels and cell adhesion molecules at the nodes of Ranvier and axonal initial segments. Regulates KCNA1 channel activity in function of dietary Mg(2+) levels, and thereby contributes to the regulation of renal Mg(2+) reabsorption (PubMed:23903368). {PubMed:17974005}.; [Isoform 5]: May be part of a Golgi-specific membrane cytoskeleton in association with beta-spectrin. {PubMed:17974005}.
• Additionnal Information: Immunoblotting: use at 1-2ug/mL. A band of >200kDa is detected.
  
  These are recommended concentrations.
  
  Enduser should determine optimal concentrations for their applications.
  
  Positive control: rat brain membranes.
• Storage Conditions: This product is stable for at least 1 year at -20°C. Freeze in multiple aliquots to avoid repeated freeze-thaw cycles.
• Specificity: This antibody recognizes human, mouse, and rat Ankyrin-G.
• Formulation: PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.Purified by Protein G affinity chromatography.
• Buffer pH: pH 7.4
• Target Background: Ankyrins are a family of adaptor proteins that mediate the attachment of integral membrane proteins to the spectrin-actin based membrane skeleton. Ankyrins have binding sites for the beta subunit of spectrin and for at least 12 families of integral membrane proteins. This linkage is required to maintain the integrity of the plasma membranes and to anchor specific ion channels, ion exchangers, and ion transporters in the plasma membrane.