Phospho-Vimentin (S39) Recombinant Rabbit Monoclonal Antibody [JE43-26]

Phospho-Vimentin (S39) Recombinant Rabbit Monoclonal Antibody [JE43-26]

• Description: Vimentin is a structural protein that in humans is encoded by the VIM gene. Its name comes from the Latin vimentum which refers to an array of flexible rods. IF proteins are found in all animal cells as well as bacteria. Intermediate filaments, along with tubulin-based microtubules and actin-based microfilaments, comprises the cytoskeleton. All IF proteins are expressed in a highly developmentally-regulated fashion; vimentin is the major cytoskeletal component of mesenchymal cells. Because of this, vimentin is often used as a marker of mesenchymally-derived cells or cells undergoing an epithelial-to-mesenchymal transition (EMT) during both normal development and metastatic progression.
• Product Name Alternative: CTRCT30 antibody Epididymis luminal protein 113 antibody FLJ36605 antibody HEL113 antibody VIM antibody VIME_HUMAN antibody Vimentin antibody
• Abbreviation: VIM
• CAS Number: 9000-83-3
• Swiss Prot: P08670 Human, P20152 Mouse, P31000 Rat
• Cellular Locus: Cytoplasm, cytoskeleton, Nucleus matrix, Cell membrane.
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Ser39 of human vimentin protein.
• Isotype: IgG
• Clone: JE43-26
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IF-Cell
• Positive Control: HeLa treated with 100nM Calyculin A for 30 minutes whole cell lysate, HeLa treated with 100nM Calyculin A for 30 minutes, NIH/3T3 treated with 100nM Calyculin A for 30 minutes cell lysate, C6 treated with 100nM Calyculin A for 30 minutes cell lysate, NIH/3T3 treated with 100nM Calyculin A for 30 minutes.
• Concentration: 1 mg/mL
• Dilution: WB: 1:1,000 ;IF-Cell: 1:100
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 54 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
• Recombinant Antibody: Yes