iFluor™ 488 Conjugated Vimentin Recombinant Rabbit Monoclonal Antibody [SC60-05]

iFluor™ 488 Conjugated Vimentin Recombinant Rabbit Monoclonal Antibody [SC60-05]

• Description: Vimentin (57 kDa) is the most ubiquituos intermediate filament protein and the first to be expressed during cell differentiation. All primitive cell types express vimentin but in most non-mesenchymal cells it is replaced by other intermediate filament proteins during differentiation. Vimentin is expressed in a wide variety of mesenchymal cell types: fibroblasts, endothelial cells etc., and in a number of other cell types derived from mesoderm, e.g., mesothelium and ovarian granulosa cells. Vimentin is present in many different neoplasms but is particulary expressed in those originated from mesenchymal cells. Sarcomas e.g., fibrosarcoma, malignt fibrous histiocytoma, angiosarcoma, and leio- and rhabdomyosarcoma, as well as lymphomas, malignant melanoma and schwannoma, are virtually always vimentin positive. Mesoderm derived carcinomas like renal cell carcinoma, adrenal cortical carcinoma and adenocarcinomas from endometrium and ovary usually express vimentin. Also thyroid carcinomas are vimentin positive. Any low differentiated or sarcomatoid carcinoma may express some vimentin. Vimentin is frequently included in the so-called primary panel (together with CD45, cytokeratin, and S-100 protein): Intense staining reaction for vimentin without coexpression of other intermediate filament proteins is strongly suggestive of a mesenchymal tumour or a malignant melanoma. However, in biopsies representing only a sarcomatoid part of renal cell carcinoma a.o. a strong positivity for vimentin without cytokeratin expression may be seen. Tumours like lymphomas and seminomas have the same intermediate filament profile, but the vimentin expression is usually weaker.
• Product Name Alternative: CTRCT30 antibody Epididymis luminal protein 113 antibody FLJ36605 antibody HEL113 antibody VIM antibody VIME_HUMAN antibody Vimentin antibody
• Abbreviation: VIM
• CAS Number: 9000-83-3
• Swiss Prot: P08670 Human, P20152 Mouse, P31000 Rat
• Cellular Locus: Cytoplasm.
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic peptide within C-terminal human Vimentin.
• Isotype: IgG
• Conjugation: iFluor™ 488
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: IF-Cell, IF-Tissue, FC
• Positive Control: C2C12, Hela, L6, human kidney tissue, human pancreas tissue, human appendix tissue.
• Concentration: 1 mg/mL
• Dilution: IF-Cell: 1:100;IF-Tissue: 1:200;FC: 1:1,000
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS.
• Molecular Weight: Predicted band size: 54 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Recombinant Antibody: Yes