FITC Conjugated CD15 Recombinant Rabbit Monoclonal Antibody [PD00-42]

FITC Conjugated CD15 Recombinant Rabbit Monoclonal Antibody [PD00-42]

• Description: CD15 is a complex cluster of cell surface glycoproteins and glycolipids having a common terminal pentasaccaharide known as the Lewisx (Lex) antigen. CD15 is a haemopoietic differentiation antigen expressed on most terminally differentiated myeloid cells including granulocytes, eosinophils, mast cells, monocytes/macrophages, and Langerhans' cells. CD15 is not substantially expressed on haemopoietic progenitor cells. The positivity for CD15 is characteristic of Hodgkin’s cells in classical Hodgkin’s disease (HD). Rare cases of acute lymphoblastic leukaemia, in which myeloid antigens are often CD15 positive. Myeloid leukaemia cells express CD15 in a heterogeneous manner. CMLs are regularly CD15 positive. CD15 is expressed in a varying proportion of epithelial tumours such as adenocarcinomas (particularly from breast, lung and colon), renal cell carcinoma, apocrine carcinoma of the skin, papillary and follicular carcinoma of the thyroid, and serous carcinoma of the ovary. It is possible that sialyl-CD15 confer on the tumour cells the capacity to metastasize. Malignant mesothelioma is practically always CD15 negative (positivity has been reported in up to 6%, particularly the desmoplastic variant). In gliomas, CD15 positivity inversely correlates with the grade of malignancy. Among germ cell tumours, CD15 is detected only in mature teratoma. In haematopathology CD15 is important for the diagnosis of classical HD and characterization of acute leukaemia. CD15 may be used for histopathological grading of gliomas and differentiating between malignant gliomas and non-neoplastic glial cells (the latter usually strongly stained). Kidney and tonsil are recommended as positive and negative tissue controls for CD15.
• Product Name Alternative: 3 fucosyl N acetyl lactosamine antibody 3-FAL antibody 3-fucosyl-N-acetyl-lactosamine antibody Leu M1 antibody Lewis X antibody LeX antibody SSEA-1 antibody stage-specific embryonic antigen 1 antibody
• Abbreviation: CD15
• CAS Number: 9000-83-3
• Swiss Prot: P22083 Human
• Cellular Locus: Golgi apparatus, Golgi stack membrane
• Host: Rabbit
• Species Reactivity: Human, Mouse
• Immunogen: Purified CD15.
• Isotype: IgG
• Clone: PD00-42
• Conjugation: FITC-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: FC
• Positive Control: Mouse peripheral blood.
• Dilution: FC: 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: Supplied in phosphate-buffered solution, pH 7.2, containing 0.2% ProClean 950 and BSA.
• Molecular Weight: Predicted band size: 59 kDa
• Storage Conditions: Store at 2°C to 8°C. Avoid repeated freeze / thaw cycles.
• Recombinant Antibody: Yes