Rabbit anti Desmin K5

Rabbit anti Desmin K5

• Background: Desmin is a 53 kDa intermediate filament protein and exhibits a high degree of tissue specificity, its expression being predominantly confined to all types of muscle cells (cardiac, skeletal and smooth muscle). Regulation of desmin expression is stage and tissue-specific, since it is induced during terminal development and muscle cell differentiation. In skeletal en cardiac muscle cells desmin is localized in the Z-disk region and at the intercalated disk. The expression pattern of desmin in smooth muscle is much more heterogenous. Coexpression of desmin and vimentin has been observed in tumors derived from muscle tissue, i.e. rhabdomyosarcomas and leiomyosarcomas. Furthermore, during myocard dysfunction dramatic changes in the distribution of desmin have been observed.
• CAS Number: 9007-83-4
• UniProt: P17661
• Host: Rabbit
• Species Reactivity: Bovine, Chicken, Caprine, Hamster, Human, Mouse, Rat, Zebrafish
• Clone: Polyclonal
• Type: Primary Antibodies
• Source: K5 is a Rabbit Serum directed anti Chicken gizzard muscle desmin. Desmin was purified from a crude tissue preparation by prepaRative gel electrophoresis as described in reference 1.
• Applications: IHC (frozen), IHC (paraffin), WB
• Field of Research: Cytoskeleton
• Assay Principle: K5 is suitable for immunoblotting and immunohistochemistry on frozen and paraffin-embedded tissues. Optimal antibody dilution should be determined by titration; recommended range is 1:25 – 1:100 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 – 1:500 for immunoblotting applications.
• Form: Each vial contains 250 ul Rabbit polyclonal antiSerum containing 0,09% sodium azide
• Precautions: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• References & Citations: 1. Ramaekers FC, Puts JJ, Moesker O, Kant A, Huysmans A, Haag D, Jap PH, Herman CJ, Vooijs GP (1983). Antibodies to intermediate filament proteins in the immunohistochemical identifiCation of Human tumours: an overview. Histochem J, 15, 691-713 _x000D_ 2. Quax W, Van den Broek L, Egberts WV, Ramaekers F, Bloemendal H (1985). Characterization of the Hamster desmin gene: expression and formation of desmin filaments in nonmuscle cells after gene transfer. Cell, 43, 327-38. _x000D_ 3. Pieper FR, Slobbe RL, Ramaekers FC, Cuypers HT, Bloemendal H (1987). Upstream regions of the Hamster desmin and vimentin genes regulate expression during in vitro myogenesis. EMBO J, 6, 3611-8. _x000D_ 4. Krimpenfort PJ, Schaart G, Pieper FR, Ramaekers FC, Cuypers HT, Van den Heuvel RM, Vree Egberts WT, van Eys GJ, Berns A, Bloemendal H (1988). Tissue-specific expression of a vimentin-desmin hybrid gene in transgenic mice. EMBO J, 7, 941-7. _x000D_ 5. van den Heuvel RM, van Eys GJ, Ramaekers FC, Quax WJ, Vree Egberts WT, Schaart G, Cuypers HT, Bloemendal H (1988). Intermediate filament formation after transfection with modified Hamster vimentin and desmin genes. J Cell Sci, 88, 475-82. _x000D_ 6. Pieper FR, Schaart G, Krimpenfort PJ, Henderik JB, Moshage HJ, van de Kemp A, Ramaekers FC, Berns A, Bloemendal H (1989). Transgenic expression of the muscle-specific intermediate filament protein desmin in nonmuscle cells. J Cell Biol, 108, 1009-24. _x000D_ 7. Schaart G, Viebahn C, Langmann W, Ramaekers F (1989). Desmin and titin expression in early postimplantation Mouse embryos. Development, 107, 585-96. _x000D_ 8. Raats JM, Pieper FR, Vree Egberts WT, Verrijp KN, Ramaekers FC, Bloemendal H (1990). Assembly of amino-terminally deleted desmin in vimentin–free cells. J Cell Biol, 111, 1971-85. _x000D_ 9. Council, L., Hameed, O. (2009). Differential expression of immunohistochemical markers in bladder smooth muscle and myofibroblasts, and the potential utility of desmin, smoothelin, and vimentin in staging of bladder carcinoma Modern Pathology 22, 639-50.
• Storage Conditions: The antibody is shipped at ambient temperature and may be stored at +4°C; For prolonged storage prepare appropriate aliquots and store at or below -20°C; Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2); Repeated thawing and freezing should be avoided; Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day; If a slight precipitation occurs upon storage, this should be removed by centrifugation; It will not affect the performance or the concentration of the product