Products for Research Use Only

Recombinant Human Neuron-specific Enolase

CAT: 0355-AP60421Size: InquireDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0355-AP60421Size:Inquire
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Background
Neuron-specific Enolase (Enolase 2) is a 47.1 kDa member of the Enolase family of enzymes. It is expressed in developing neurons and glia, is known to catalyze the generation of phosphoenolpyruvate, and is suggested to possess neurotrophic activity for neurons, likely through an extracellular mechanism. Human Neuron-specific Enolase is 433 amino acids (aa) in length. The enzymatic site spans most of the length of the molecule. Neuron-specific Enolase exists as both a noncovalently-linked homodimer, or heterodimer with alpha-enolase. Full-length human Enolase 2 shares 99% aa identity with both murine and canine Enolase 2. It shares 83 % aa identity with human enolases 1 and 3.
Target
SIEKIWAREI LDSRGNPTVE VDLYTAKGLF RAAVPSGAST GIYEALELRD GDKQRYLGKG VLKAVDHINS TIAPALISSG LSVVEQEKLD NLMLELDGTE NKSKFGANAI LGVSLAVCKA GAAERELPLY RHIAQLAGNS DLILPVPAFN VINGGSHAGN KLAMQEFMIL PVGAESFRDA MRLGAEVYHT LKGVIKDKYG KDATNVGDEG GFAPNILENS EALELVKEAI DKAGYTEKIV IGMDVAASEF YRDGKYDLDF KSPTDPSRYI TGDQLGALYQ DFVRDYPVVS IEDPFDQDDW AAWSKFTANV GIQIVGDDLT VTNPKRIERA VEEKACNCLL LKVNQIGSVT EAIQACKLAQ ENGWGVMVSH RSGETEDTFI ADLVVGLCTG QIKTGAPCRS ERLAKYNQLM RIEEELGDEA RFAGHNFRNP SVL
Purification
> 96 % by SDS-PAGE and HPLC analyses.
Endotoxin
Less than 0.1 EU/ug of rHuNSE as determined by LAL method.
Bioactivity
Data Not Available.
Storage Conditions
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculated Molecular Weight
Approximately 47.1 kDa, a single non-glycosylated polypeptide chain containing 433 amino acids.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, with 5 % Trehalose.
Host or Source
Escherichia coli