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Anti-E2F-1 Antibody

CAT: 0014-3334-1Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0014-3334-1Size:100 µg
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Description
Mouse Monoclonal Antibody specific to E2F-1
CAS Number
9007-83-4
Product Name Alternative
E2F-1, PBR3, Retinoblastoma-associated protein 1, RBAP-1, Retinoblastoma-binding protein 3, RBBP-3, pRB-binding protein E2F-1
Gene Name
E2F1
Gene ID
1869
NCBI Gene ID
E2F1
UniProt
Q01094
Accession Number
NP_005216
Cellular Locus
Nucleus
Host
Mouse
Reactivity
Human
Immunogen
GST fusion protein expressed in E. coli corresponding to aa 162-476 of human E2F-1.
Target Antigen
Transcription factor E2F1
Target
E2F-1
Clonality
Monoclonal
Isotype
IgG1
Type
Antibody
Applications
WB, ICC/IF, IP, IHC
Field of Research
Cancer Research
Purification Method
Purified by Protein G affinity chromatography
Concentration
Lot Specific
Dilution
Dilute in PBS or medium which is identical to that used in the assay system.
Format
Purified
Form
Liquid
Buffer
Phosphate Buffered Saline
Function
Transcription activator that binds DNA cooperatively with DP proteins through the E2 rPubMed:10675335, PubMed:12717439, PubMed:17050006, PubMed:17704056, PubMed:18625225, PubMed:20040599, PubMed:20176812, PubMed:28992046, PubMed:8170954}.
Additionnal Information
Immunoblotting: use at 1-5 ug/mL.

Immunoprecipitation: use at 1-5 ug/mL.

Positive controls: E2F-1 transfected Soas2 cells and recombinant fusion protein.
Storage Conditions
This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze- thaw cycles.
Specificity
This antibody recognizes an epitope within aa 244-264 of human E2F-1.
Formulation
PBS, pH 7.4
Buffer pH
pH 7.4

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