CD276 Antibody / B7-H3

• Description:

  T cell activation and immune function are regulated by the innate immune system through positive and negative costimulatory molecules. One such molecule, B7-H3 (B7-homolog 3, also designated B7RP-2 and CD276) belongs to the B7 immunoglobulin superfamily. Soluble B7-H3/CD276 binds a putative receptor on activated T-cells that is distinct from CD28, CTLA-4, ICOS and PD-1. Widely expressed on nonlymphoid tissues, it costimulates proliferation of both CD4+ and CD8+ T cells. The ability of B7-H3/CD276 to stimulate Th1 and cytotoxic-T cell responses suggest that it may have antitumor activity. B7-H3 interactions may play a role in regulating cell-mediated immune responses against cancer, implicating B7-H3/CD276 as a potential therapeutic tool.

• Specifications:

  Immunohistochemistry (FFPE) : 1-2 µg/mL

• UniProt:

  Q5ZPR3

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 100-300 from the human CD276 protein was used as the immunogen for the CD276 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  B7H3/4345

• Applications:

  IHC-P

• Purity:

  Protein A/G affinity

• Format:

  Purified

• Buffer:

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution:

  Aliquot the CD276 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This CD276 antibody is available for research use only.

• Storage Conditions:

  Aliquot the CD276 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes:

  Optimal dilution of the CD276 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cell surface, cytoplasm

• Image Legend:

  IHC staining of FFPE human ovarian carcinoma tissue with B7-H3 antibody (clone B7H3/4345) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.