B7-H3 Antibody / CD276

• Description:

  T cell activation and immune function are regulated by the innate immune system through positive and negative costimulatory molecules. One such molecule, B7-H3 (B7-homolog 3, also designated B7RP-2) belongs to the B7 immunoglobulin superfamily. Soluble B7-H3 binds a putative receptor on activated T-cells that is distinct from CD28, CTLA-4, ICOS and PD-1. Widely expressed on nonlymphoid tissues, B7-H3 costimulates proliferation of both CD4+ and CD8+ T cells. The ability of B7-H3 to stimulate Th1 and cytotoxic-T cell responses suggest that it may have antitumor activity. B7-H3 interactions may play a role in regulating cell-mediated immune responses against cancer, implicating B7-H3 as a potential therapeutic tool.

• Specifications:

  Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

• UniProt:

  Q5ZPR3

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  A recombinant partial protein sequence (within amino acids 100-300) from the human protein was used as the immunogen for the B7-H3 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  B7H3/4479

• Applications:

  IHC-P

• Purity:

  Protein A/G affinity

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Aliquot the B7-H3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This B7-H3 antibody is available for research use only.

• Storage Conditions:

  Aliquot the B7-H3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the B7-H3 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cytoplasm, Cell surface

• Image Legend:

  IHC staining of FFPE human tonsil tissue with B7-H3 antibody (clone B7H3/4479) at 2ug/ml. Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.