PDIA6 Antibody / ERp5

• Description:

  This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, two catalytically active thioredoxin (TRX) domains, a TRX-like domain, and a C-terminal ER-retention sequence. This protein inhibits the aggregation of misfolded proteins and exhibits both isomerase and chaperone activity. Alternative splicing results in multiple transcript variants encoding different isoforms.

• Specifications:

  Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells

• UniProt:

  Q15084

• Host:

  Mouse

• Reactivity:

  Human, Monkey

• Immunogen:

  Recombinant human protein (amino acids L20-L440) was used as the immunogen for the PDIA6 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2b

• Clone:

  3H5E7

• Applications:

  WB, IHC-P, IF, FACS

• Purity:

  Antigen affinity purified

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2% Trehalose

• Reconstitution:

  After reconstitution, the PDIA6 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This PDIA6 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the PDIA6 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the PDIA6 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic, cell membrane

• Image Legend:

  IHC staining of FFPE human breast infiltrating ductal carcinoma tissue with PDIA6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.