Prothrombin Antibody

• Description :

  F2 (Coagulation Factor II), also known as thrombin, is a serine protease that in humans is encoded by the F2 gene. This gene for human prothrombin (F2) was assigned to chromosome 11p11-q12 by analysis of a panel of somatic cell hybrid DNAs and by in situ hybridization, using both cDNA and genomic probes. The activated thrombin enzyme plays an important role in hemostasis and thrombosis: it converts fibrinogen to fibrin for blood clot formation, stimulates platelet aggregation, and activates coagulation factors V, VIII (F8), and XIII (F13A1) . Thrombin also inhibits coagulation by activating protein C.

• Specifications :

  Western blot: 0.5-1 µg/mL

• UniProt :

  P00734

• Host :

  Mouse

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  Recombinant human protein (amino acids Y97-R124) was used as the immunogen for the Prothrombin antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG1

• Clone :

  5B6G1

• Applications :

  WB

• Purity :

  Antigen affinity purified

• Format :

  Antigen affinity purified

• Buffer :

  Lyophilized from 1X PBS with 2% Trehalose

• Reconstitution :

  After reconstitution, the Prothrombin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations :

  This Prothrombin antibody is available for research use only.

• Storage Conditions :

  After reconstitution, the Prothrombin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation :

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes :

  Optimal dilution of the Prothrombin antibody should be determined by the researcher.

• CAS Number :

  9007-83-4

• Image Legend :

  Western blot testing of 1) human HeLa, 2) human HepG2, 3) human Caco-2, 4) human HL-60, 5) rat liver, 6) rat RH35, 7) mouse liver and 8) mouse HEPA1-6 cell lysate with Prothrombin antibody. Predicted molecular weight ~70 kDa but may be observed at higher molecular weights due to glycosylation.