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PPARG Antibody / PPAR gamma

CAT: 0800-RQ6963Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ6963Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Peroxisome proliferator- activated receptor gamma (PPAR-gamma or PPARG), also known as the glitazone reverse insulin resistance receptor, or NR1C3 (nuclear receptor subfamily 1, group C, member 3) is a type II nuclear receptor (protein regulating genes) that in humans is encoded by the PPARG gene. This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cells, Immunofluorescence: 5 µg/mL, Direct ELISA: 0.1-0.5 µg/mL
UniProt
P37231
Host
Rabbit
Reactivity
Human
Immunogen
Recombinant human protein (amino acids H58-K329) was used as the immunogen for the PPARG antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, FACS, IF, Direct ELISA
Purity
Antigen affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the PPARG antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This PPARG antibody is available for research use only.
Storage Conditions
After reconstitution, the PPARG antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the PPARG antibody should be determined by the researcher.
Image Legend
Flow cytometry testing of human A431 cells with PPARG antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= PPARG antibody.

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