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PPARA Antibody / PPAR alpha

CAT: 0800-F54334-0.4MLSize: 0.4 mLDry Ice: NoHazardous: No
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CAT#:0800-F54334-0.4MLSize:0.4 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl- 2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC) . Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety (By similarity) . Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2.
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1:25, Immunofluorescence: 1:25, Flow cytometry: 1:25 (1x10e6 cells), Western blot: 1:500-1:2000
UniProt
Q07869
Host
Mouse
Reactivity
Human, Mouse
Immunogen
A recombinant human protein was used as the immunogen for the PPARA antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
1331CT894.186.143
Applications
IHC-P, IF, FACS, WB
Purity
Protein G affinity
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the PPARA antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This PPARA antibody is available for research use only.
Storage Conditions
Aliquot the PPARA antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
The stated application concentrations are suggested starting points. Titration of the PPARA antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Nuclear
Image Legend
IHC testing of FFPE human skeletal muscle tissue with PPARA antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Product Datasheet
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