Phospho-Ezrin (T567)/Radixin (T564)/Moesin (T558) Recombinant Rabbit Monoclonal Antibody [PSH05-73]

Phospho-Ezrin (T567)/Radixin (T564)/Moesin (T558) Recombinant Rabbit Monoclonal Antibody [PSH05-73]

• Description: The ezrin, radixin, and moesin (ERM) proteins function as linkers between the plasma membrane and the actin cytoskeleton and are involved in cell adhesion, membrane ruffling, and microvilli formation . ERM proteins undergo intra or intermolecular interaction between their amino- and carboxy-terminal domains, existing as inactive cytosolic monomers or dimers. Phosphorylation at a carboxy-terminal threonine residue (Thr567 of ezrin, Thr564 of radixin, Thr558 of moesin) disrupts the amino- and carboxy-terminal association and may play a key role in regulating ERM protein conformation and function. Phosphorylation at Thr567 of ezrin is required for cytoskeletal rearrangements and oncogene-induced transformation. Ezrin is also phosphorylated at tyrosine residues upon growth factor stimulation. Phosphorylation of Tyr353 of ezrin transmits a survival signal during epithelial differentiation.
• Product Name Alternative: Villin 2 ezrin antibody CVIL antibody CVL antibody Cytovillin 2 antibody Cytovillin antibody DKFZp762H157 antibody Epididymis secretory protein Li 105 antibody EZR antibody EZRI_HUMAN antibody Ezrin antibody FLJ26216 antibody HEL S 105 antibody MGC1584 antibody p81 antibody VIL 2 antibody VIL2 antibody Villin 2 (ezrin) antibody Villin 2 antibody Villin-2 antibody Villin2 antibody CB567 antibody CG12537 antibody DFNB24 antibody ESP10 antibody Hh-induced MATH and BTB domain-containing protein antibody HIB antibody Moesin-B antibody Protein roadkill antibody RADI_HUMAN antibody Radixin antibody RDX antibody Epididymis luminal protein 70 antibody HEL70 antibody Membrane organizing extension spike protein antibody Membrane-organizing extension spike protein antibody MOES_HUMAN antibody Moesin antibody Moesin/anaplastic lymphoma kinase fusion protein antibody Msn antibody MSN/ALK fusion antibody
• Abbreviation: Ezrin Cytovillin Villin-2 p81 EZR VIL2 Radixin RDX Moesin Membrane-organizing extension spike protein MSN
• CAS Number: 9000-83-3
• Swiss Prot: P15311 Human, P26038 Human, P35241 Human, P26040 Mouse, P26041 Mouse, P26043 Mouse, P31977 Rat, O35763 Rat
• Cellular Locus: Cell membrane. Cell projection. Cytoplasm. Cytoskeleton. Membrane
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Thr567 of human ezrin protein.
• Isotype: IgG
• Clone: PSH05-73
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IF-Cell, IHC-P
• Positive Control: HeLa starved for 3 hours then add 100nM Calyculin A for 30 minutes cell lysate, NIH/3T3 starved for 24 hours then add 100nM Calyculin A for 30 minutes cell lysate, C6 treated with 100ng/mL Calyculin A for 1 hours cell lysate, mouse liver tissue, rat liver tissue.
• Concentration: 1 mg/mL
• Dilution: WB: 1:2,000 ;IF-Cell: 1:100 ;IHC-P: 1:1,000
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 69 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
• Recombinant Antibody: Yes