GPX4 (U73C) FLAG-Tag Recombinant

CAT: 0421-100543Size: 100 µgDry Ice: YesHazardous: No

1 / 1

CAT#:0421-100543Size:100 µg

Selected

24/48H Stock Items & 2 to 6 Weeks non Stock Items.

Quick Request Actions

Background

GPX4, also known as glutathione peroxidase 4, is one of the eight isoenzymes of the glutathione peroxidase family. It is a monomer and uses selenium as co-factor. GXP4 works as a phospholipid hydroperoxidase in protecting cell membranes from lipid peroxidation. It uses reduced glutathione during the reduction of hydrogen peroxide, hydroperoxides and lipid peroxides. The synthesis of GXP4 is metabolically costly to cells, and it seems to be regulated by SLC7A11 (cystine/glutamate transporter) -mediated cystine uptake and activation of mTORC1 (mammalian target of rapamycin complex 1) . The U73C mutation has been shown to reduce enzyme activity. A mice knock-out model indicated the importance of this protein in embryonic development and lifespan. In humans, lack of GPX4 activity results in ferroptosis and spondylometaphyseal dysplasia. Its role in ferroptosis and link to mTOR1 make studies into GXP4 of interest in the field of cancer therapy. Further studies will elucidate its role in more detail and may provide therapeutic opportunities.

Description

Recombinant human GPX4 (glutathione peroxidase 4), encompassing amino acids 29-197 (end) . This construct contains an N-terminal FLAG-tag. This protein contains the mutation U73C. The recombinant protein was affinity purified.

CAS Number

9000-83-3

UniProt

P36969

Tag

N-terminal FLAG-tag

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Concentration

0.30 mg/mL

Purity

≥ 90%

Format

Aqueous buffer solution

Shipping Conditions

-80°C

Storage Conditions

At least 6 months at -80°C.

Calculated Molecular Weight

21 kDa

Formulation

40 mM Tris-HCl, pH 8.0,110 mM NaCl, 2.2 mM KCl, 100 µg/ml FLAG peptide, and 20% glycerol

UniProtKB · P36969

Phospholipid hydroperoxide glutathione peroxidase GPX4

GPX4_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: P36969
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: GPX4
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: PHGPx
EC number: 1.11.1.12
Processing: Precursor
Secondary accessions: O43381, Q6PJ59, Q9UPK2

Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative initiationSelenocysteine

Cellular component

CytoplasmMitochondrion

Molecular function

Developmental proteinOxidoreductasePeroxidase

Disease

Dwarfism

PTM

HydroxylationPhosphoprotein

Biological process

Lipid metabolism

Domain

Transit peptide

Related Products

Catalog	Name
102647	GPX4, His-Tag Recombinant
100057	ALKBH5, FLAG-Tag Recombinant
100069	SARM1, FLAG-Tag Recombinant
100472-1	ADAR1, FLAG-Tag Recombinant
50161-1	TET1, FLAG-tag Recombinant
91003	ANGPT2, FLAG-tag Recombinant
71205	LDLR, FLAG-tag Recombinant
50161-2	TET1, FLAG-tag Recombinant
100472-2	ADAR1, FLAG-Tag Recombinant
100255-2	Cereblon, FLAG-Tag Recombinant

Alternative Products

Catalog	Name
HY-P703942	GPX4, Human (U73C, HEK293, Flag)
GPX4-1895H	Recombinant Human GPX4 Protein, Flag-tagged
MBS2580210-01	Recombinant Human GPX4 Protein (Sumo Tag)
MBS8125861-01	GPX4 Protein, Human, Recombinant (His Tag)
373520-01	GPX4, Recombinant, Pongo pygmaeus, aa1-170, His-SUMO-Tag (Phospholipid Hydroperoxide Glutathione Peroxidase, Mitochondrial)
ARP2324-01	Recombinant Human GPX3 (U73C) Protein
TMPH-03938-01	GPX3 Protein, Human, Recombinant (U73C, hFc)
PPL01669-4c	PLVX-AcGFP1-C1-GPX4-3*Flag
PVT56353	pCMV-GPX4-3×FLAG-Neo Plasmid
P98530	PRK5-CMV-FLAG-GPX4 (human) -SECIS