Human ITAC (CXCL11) Recombinant

• Background:

  The CXC chemokine ligand 11 (CXCL11) or IFN-inducible T-cell-chemoattractant (I-TAC) belongs to the CXC chemokine family characterized by the presence of 1 amino acid in between the 2 NH2-terminal cysteines. CXCL11 is produced by a variety of cells including leukocytes, fibroblasts, and endothelial cells upon stimulation with interferons (IFNs) . Simultaneous stimulation of fibroblasts or endothelial cells with IFN-gamma and interleukin-1b or the TLR3 ligand double-stranded RNA resulted in a synergistic increase of CXCL11 production. CXCL11 attracts activated T-helper 1 (Th1) lymphocytes and natural killer (NK) cells. Like CXCL9 and CXCL10, CXCL11 signals through CXC chemokine receptor 3 (CXCR3) .

• Description:

  Recombinant human CXCL11 (ITAC) is a disulfide-linked monomeric protein consisting of 74 amino acid residues and migrates as an approximately 8 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human ITAC (CXCL11) mature chain was expressed inE. coli.

• UniProt:

  O14625

• Endotoxin:

  < 0.1 ng/µg (1 EU/µg), using the LAL gel clot method.

• Purity:

  ≥ 98% by SDS-PAGE and HPLC

• Format:

  Lyophilized protein

• Reconstitution:

  Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.

• Shipping Conditions:

  Gel Packs or Dry Ice

• Storage Conditions:

  The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.

• Calculated Molecular Weight:

  8 kDa

• Formulation:

  Lyophilized from a 0.2 µm filtered 20 mM Phosphate buffer, 100 mM NaCl, pH 7.5.