Human Insulin Recombinant

• Description:

  Recombinant human Insulin is a disulfide-linked homodimeric protein consisting of 51 amino acid residues, and migrates as an approximately 6 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human Insulin mature chain was expressed inE. coli.

• UniProt:

  P01308

• Endotoxin:

  Endotoxin level was found to be < 0.1 ng/µg (1 EU/µg), using the LAL gel clot method.

• Purity:

  ≥ 95% by SDS-PAGE and HPLC

• Format:

  Lyophilized protein

• Reconstitution:

  A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/ml. This solution can then be diluted into other buffers.

• Shipping Conditions:

  Gel Packs or Dry Ice

• Storage Conditions:

  The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.

• Calculated Molecular Weight:

  6 kDa

• Formulation:

  Lyophilized from a 0.2 µm filtered aqueous solution with no additives