Human Interleukin-7 Recombinant

• Background:

  IL-7 is secreted constitutively into the conditioned medium of adherent bone marrow stromal cells and thymic cells. Mouse and human keratinocytes have been shown also to express and secrete IL-7. Human (152 amino acids) and murine IL-7 (129 amino acids) show 60% sequence homology at the protein level. The human IL-7 receptor, designated CD127, is an integral strongly glycosylated membrane protein of 76 kDa expressed on activated T-cells. IL-7 receptors are expressed on pre-B-cells and their progenitors and bone marrow macrophages, but they are not expressed on mature B-cells. Functional IL-7 receptors are also found on the cell surface of multiphenotypic, biphenotypic, and immature lymphoid progenitors of B-cells with the gene arrangement of the heavy immunoglobulin chain such as those observed in the germ line.

• Description:

  Recombinant human IL-7 is a disulfide-linked monomeric protein consisting of 153 amino acid residues, and migrates as an approximately 17 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human Interleukin-7 mature chain was expressed inE. coli.

• UniProt:

  P13232

• Endotoxin:

  < 0.1 ng/µg (1 EU/µg), using the LAL gel clot method.

• Purity:

  ≥ 97% by SDS-PAGE and HPLC

• Format:

  Lyophilized protein

• Reconstitution:

  Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.

• Shipping Conditions:

  Gel Packs or Dry Ice

• Storage Conditions:

  The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.

• Calculated Molecular Weight:

  17 kDa

• Formulation:

  Lyophilized from a 0.2 µm filtered PBS solution, pH 4.5