Human Interleukin-6 Recombinant

• Background:

  IL-6 is a member of a family of cytokines, which also includes LIF, CNTF, Oncostatin M, IL11, and CT- 1. All known members of the IL-6 cytokine family induce hepatic expression of acute phase proteins. IL-6 is produced by many different cell types. The main sourcesin vivoare stimulated monocytes, fibroblasts, and endothelial cells. Macrophages, T-cells and B-lymphocytes, granulocytes, smooth muscle cells, eosinophils, chondrocytes, osteoblasts, mast cells, glial cells, and keratinocytes also produce IL-6 after stimulation. The IL-6 receptor is expressed on T-cells, mitogen-activated B-cells, peripheral monocytes and some macrophage- and B-cell derived tumor cell types. It is not expressed in resting B-cells but in resting T-cells. The IL-6 receptor (CD126) is a strongly glycosylated protein of 80 kDa and a length of 449 amino acids.

• Description:

  Recombinant human Interleukin-6, also known as IL-6, is a disulfide-linked homodimeric protein consisting of 183 amino acids and migrates as an approximately 20 kDa protein under reducing conditions.

• UniProt:

  P05231

• Endotoxin:

  < 0.1 ng/µg (1 EU/µg), using the LAL gel clot method.

• Purity:

  ≥ 97% by SDS-PAGE and HPLC

• Format:

  Lyophilized protein

• Reconstitution:

  A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/ml. This solution can then be diluted into other buffers.

• Shipping Conditions:

  Gel Packs or Dry Ice

• Storage Conditions:

  The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.

• Calculated Molecular Weight:

  20 kDa

• Formulation:

  Lyophilized from a 0.2 µm filtered PBS solution, pH 7.0.