Human Interleukin-29 Recombinant

• Background:

  The IFN-lambda gene family is composed of three distinct genes: IFN-lambda1 (IL-29), IFN-lambda2 (IL- 28A), and IFN-lambda3 (IL- 28B) . IFN-lambda proteins exhibit only weak homology to IL-10, but, like IL-10, they also use the IL-10R2 chain as a component of their receptor complex. The IFN-receptor complex consists of the unique ligand-binding chain IFN- R. The IFN-λ1 gene is found exclusively in primate genomes and is 81% identical to IFN-λ2/3. IFN-λs, which are now collectively referred to as type III IFNs, exhibit several features in common with type I IFNs, such as antiviral, antiproliferative, and antitumor activities.

• Description:

  Recombinant Interleukin-29 (IFN-lambda) is a disulfide-linked monomer protein consisting of 178 amino acid residues, and migrates as an approximately 20 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding murine Interleukin-29 mature chain was expressed inE. coli.

• UniProt:

  Q8IU54

• Endotoxin:

  < 0.1 ng/µg (1 EU/µg), using the LAL gel clot method.

• Purity:

  ≥ 95% by SDS-PAGE and HPLC

• Format:

  Lyophilized protein

• Reconstitution:

  Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.

• Shipping Conditions:

  Gel Packs or Dry Ice

• Storage Conditions:

  The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.

• Calculated Molecular Weight:

  20 kDa

• Formulation:

  Lyophilized from 0.2 µm filtered solution in 130 mM NaCl, 20 mM phosphate buffer, pH 7.5.