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PARPtrap™ Assay Kit for PARP2

CAT: 0421-78296-1Size: 96 ReactionsDry Ice: YesHazardous: No
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CAT#:0421-78296-1Size:96 Reactions
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The PARPtrap™ Assay Kit for PARP2 is a fluorescence polarization (FP), homogeneous, 96-well or 384-well assay designed for screening and profiling small molecules that trap PARP2 onto DNA and complex formation in a high throughput screening format. The PARPtrap™ Assay Kit for PARP2 comes in a convenient 96-well format, with purified recombinant PARP2 enzyme (amino acids 2-583), a fluorescent-labeled oligonucleotide duplex, NAD+and PARPtrap™ assay buffer for 100 or 400 enzyme reactions.Figure 1. Diagram illustration the mode of action of PARPtrap™ Assay Kit for PARP2.The fluorescent probe is a small molecule that can rotate freely in solution, resulting in low FP. In the absence of ribosylation, PARP2 binds to the fluorescent probe, forming a large complex and resulting in the emission of highly polarized light. After the auto-ribosylation step, PARP2 dissociates from the fluorescent oligonucleotide duplex, which can then rotate freely again (low FP) . In the presence of a PARP2 inhibitor, PARP2 is trapped to the fluorescent oligonucleotide duplex, having a high FP. The increase in FP signal is directly proportional to PARP trapping.This assay requires a fluorescent microplate reader capable of measuring fluorescence polarization (FP) and equipped with the required parts to read the FP signal. For more information FP technology, visit our Tech Note:FP, assay principles and applications.Need us to run inhibitor screens or profile your compounds against PARPtrap™ for PARP2? Check out ourPARP/PARPTrap™ Screening ServicesorDNA Replication and Repair Screening Services.
UniProt
Q9UGN5
Applications
Screen small molecules that enhance PARP2/DNA trapping for drug discovery and high throughput screening (HTS) applications, and IC50determination.
Format
96 ReactionsCatalog #NameAmountStorage80502PARP2, GST-tag*8 µg-80°C78297100 nM Fluorescent Labeled Nicked Oligonucleotide Duplex12.5 µl-80°C5x PARPtrap™ Assay Buffer 22 x 1 ml-80°C10x NAD+500 µl-80°C0.5 M DTT200 µl-20°C79685Black 96-well plate1Room Temp* The initial concentration of enzyme is lot-specific and will be indicated on the tube containing the protein.384 ReactionsCatalog #NameAmountStorage80502PARP2, GST-Tag*2 x 8 µg-80°C78297100 nM Fluorescent Labeled Nicked Oligonucleotide Duplex4 x 12.5 µl-80°C5x PARPtrap™ Assay Buffer 24 x 1 ml-80°C10x NAD+2 x 500 µl-80°C0.5 M DTT2 x 200 µl-20°C79961Black 384-well plate1Room Temp*The initial concentration of enzyme is lot-specific and will be indicated on the tube containing the protein.
Shipping Conditions
-80°C
Storage Conditions
This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Notes
Troubleshooting GuideVisitbpsbioscience.com/assay-kits-faqfor detailed troubleshooting instructions. For all further questions, please email[email protected]
Contraindications
The final concentration of DMSO in the assay should not exceed 1%.Fluorescent compounds that have λex=470 (5 nm bandwidth) and detection at λem=518 (10 nm bandwidth) can interfere with the readouts.

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