PARP2 Colorimetric Assay Kit

PARP2, also known as poly- (ADP-ribose) polymerase 2 or NAD+ADP-ribosyltransferase 2, is part of the PARP family.  ADP ribosylation, which is the addition of an ADP-ribose to a protein, is a reversible post-translational modification of proteins mostly involved in the DNA Damage Response (DDR) pathway. Poly-ADP-ribosylation (termed PARylation) is the addition of linear or branched chains of ADP-ribose. PARP2 participates in DNA repair (only 10% of the PARP activity is due to PARP2), but also in oxidative stress and mitochondrial fragmentation. Dysfunction of the DDR and oxidative stress pathways can lead to oncogenesis. Genetic ablation of PARP2 has indicated roles of PARP2 in adipogenesis, spermatogenesis and thymocyte survival. It is also a co-factorofnuclear receptors like ER (estrogen receptor) and PPAR (peroxisome proliferator-activated receptors) . PARP2 is overexpressed in prostate cancer (PCa) and may contribute to the disease through the FOXA1 (forkhead box protein A1) /AR pathway. PARP inhibitors have been used in cancer treatment with success, with the clinically approved inhibitors targeting both PARP1 and PARP2. Further understanding of the molecular pathways involving PARP2, anditscontribution to disease, will continue to pave the way for new therapies for PARP2-linked diseases.

The PARP2 Colorimetric Assay Kit is designed to measure PARP2 (poly- (ADP-ribose) polymerase 2) activity for screening and profiling applications. The PARP2 assay kit comes in a convenient 96-well format, with enough purified PARP2 enzyme (amino acids 2-583), reaction substrates and PARP assay buffer for 100 enzyme reactions.Figure 1. PARP2 Colorimetric Assay Kit schematic.Histone proteins are coated on a 96-well plate. Next, a biotinylated NAD+mix (termed PARP Substrate Mixture) is incubated with the PARP2 enzyme and an activated DNA template in an optimized assay buffer. The plate is then treated with streptavidin-HRP followed by addition of the colorimetric HRP Substrate which generates a colored solution.  The signal generated is proportional to PARP2 activityNeed us to run inhibitor screens or profile your compounds against PARP2? Check out ourPARP/PARPTrap™ Screening ServicesorDNA Replication and Repair Screening Services.This product has beencited 12 times.

Q9UGN5

Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput (HTS) applications.

Catalog #NameAmountStorage80502PARP2, GST-Tag*3 µg-80°C520295x Histone Mixture1 ml-80°C78366PARP Substrate Mixture 12 x 250 µl-80°C8060210x PARP Assay Buffer1 ml-20°C79743Blocking Buffer 325 ml+4°C80605Activated DNA20 µl-80°C80611Streptavidin-HRP100 µl+4°C79651Colorimetric HRP Substrate10 ml+4°C0.5 M DTT200 µl-20°C7996496-well transparent plate1Room Temp*The concentration of the protein is lot-specific and will be indicated on the tube

-80°C

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

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