Fluorogenic Rat HDAC6 Assay Kit

• Background:

  Histone Deacetylases (HDACs) catalyze the hydrolysis of an acetyl group from acetyl-lysine present in histones. Histone acetylation/deacetylation status tightly regulates transcription. HDAC6 plays a role in stress granule formation, cell motility, and leptin sensitivity. HDAC6 interacts with heat-shock protein 90 (HSP90) and with ubiquitinated proteins and has been linked to Alzheimer’s disease and tumor growth and metastasis. The use of selective inhibitors for HDAC6 offers potential benefits for neurodegenerative diseases and cancer treatment.

• Description:

  The rat histone deacetylase 6 (HDAC6) Assay Kit is a 96-well fluorogenic assay designed to measure rat HDAC6 enzymatic activity for screening and profiling applications. In addition, the kit includes a potent HDAC inhibitor, Trichostatin A (TSA), for use as control.Figure 1: Illustration of the principle behind the Fluorogenic Rat HDAC Assay Kit.The Fluorogenic Rat HDAC6 Assay Kit is based on a unique fluorogenic substrate and developer combination. A fluorescent dye is conjugated to a peptide containing acetyl-lysine. The fluorescence of the conjugated dye is quenched by the peptide. After treatment of the peptide with an HDAC, a developer solution specific for non-acetylated lysine is added to the reaction. If the acetyl group has been removed from the lysine, the developer releases the dye, allowing for fluorescence. In this assay, fluorescence directly correlates with HDAC activity.Need us to run inhibitor screens or profile your compounds against Rat HDAC6? Check out ourDeacetylase/Sirtuin Screening Services.

• UniProt:

  A0A0G2QC41

• Applications:

  Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.

• Format:

  Catalog #NameAmountStorage101332HDAC6, GST-His-Tag (Rat) *9 µg-80°C50037Fluorogenic HDAC substrate 3 (5 mM) 25 µl-80°C500302x HDAC Developer (contains Trichostatin A) (2 µM) 6 ml-80°CTrichostatin A in DMSO (200 µM) **100 µl-20°C50031HDAC Assay Buffer10 ml-20°C79685Black 96-well plate1Room Temp*The concentration of the protein is lot-specific and will be indicated on the tube.**HDAC6 inhibitor Trichostatin A is provided as an internal control for HDAC6 activity.

• Shipping Conditions:

  -80°C

• Storage Conditions:

  This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

• Notes:

  Troubleshooting GuideVisitbpsbioscience.com/assay-kits-faqfor detailed troubleshooting instructions. For further questions, please email[email protected]