Spike Trimer (S1+S2) (B.1.351, Beta Variant, Δ242-244) (SARS-CoV-2) Recombinant

• Description:

  Recombinant SARS-CoV-2 Spike protein in its homotrimeric form, containing S1+S2 subunits and encompassing amino acids 16-1213. This protein corresponds to SARS-CoV-2 Variant B.1.351, also known as variant Beta originally discovered in South Africa, and contains the nine known mutations listed below, in addition to deletion 242-244*. The construct also contains mutations 682RRAR685>A, K986P and V987P, and a T4 trimerization domain followed by a His-tag (6xHis) in C-terminal. The recombinant protein was affinity purified.*Deletion 242-244 is known as 241-243 in some databases and on the CDC website. This is the same deletion. As stated byTegally et al., Nature 2021: "We also observe a deletion of three amino acids at positions 242 to 244, which was seen in samples extracted and generated in different laboratories across the NGS-SA. This region is difficult to align; the deletion could potentially also be located at positions 241 to 243, but the resulting sequence would be exactly the same. "This product has beencited 3 times.

• UniProt:

  P0DTC2

• Tag:

  C-terminal His-tag

• Purity:

  ≥ 90%

• Format:

  Aqueous buffer solution

• Shipping Conditions:

  -80°C

• Storage Conditions:

  At least 6 months at -80°C.

• Calculated Molecular Weight:

  137 kDa + glycans

• Formulation:

  8 mM phosphate, pH 7.4,110 mM NaCl, 2.2 mM KCl, 20% glycerol

• Glycosylation:

  This protein runs at a higher molecular weight due to glycosylation.