Spike Trimer (S1+S2), His-tag, Eu-labeled (SARS-CoV-2) Recombinant

• Description:

  Recombinant SARS-CoV-2 Spike protein in its homotrimeric form, containing S1+S2 subunits and encompassing amino acids 16-1213. This protein contains mutations 682RRAR685>A, K986P and V987P. The construct also contains a C-terminal His tag (6xHis) . The recombinant protein was affinity purified and labeled with trFluor-Eu.

• Tag:

  C-terminal His-tag

• Label:

  TrFluor™ Eu (Ex/Em = 346nm / 617 nm) is a fluorophore with large Stokes shifts and an extremely long emission half-live. It is ideal for applications that require high sensitivity and for time-resolved fluorometry because a delay between excitation and the detection of emission minimizes interference. EU-labeled probes are characterized by a relatively high stability and are often insensitive to quenching. trFluor™ Eu is covalently and randomly conjugated to the target protein.

• Applications:

  Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

• Purity:

  ≥ 90%

• Shipping Conditions:

  -80°C

• Storage Conditions:

  At least 6 months at -80°C.

• Calculated Molecular Weight:

  139 kDa + glycans

• Formulation:

  40 mM Tris, pH 8.0,110 mM NaCl, 2.2 mM KCl, 200 mM Imidazole and 20% glycerol.

• Glycosylation:

  This protein runs at higher MW by SDS-PAGE due to glycosylation.