SARS-CoV-2 Spike Trimer (S1+S2) :ACE2 Inhibitor Screening Colorimetric Assay Kit

• Background:

  The pandemic coronavirus disease 2019 (COVID-19) is caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) . As a first step of the viral replication strategy, the virus attaches to the host cell surface before entering the cell. The Receptor Binding Domain (RBD) of Spike protein recognizes and attaches to the Angiotensin-Converting Enzyme 2 (ACE2) receptor found on the surface of type I and II pneumocytes, endothelial cells, and ciliated bronchial epithelial cells. It has been widely suggested that active as well as passive immunizations targeting the interaction between the Spike protein of SARS-CoV-2 and ACE2 offer promising protection against the viral infection. However recent reports showed that a mutant strain first identified in the UK (B.1.1.7) exhibits higher transmissibility and infectivity. Investigations on the effects of mutations on viral replication and pathogenesis will be critical for developing effective strategies for vaccines and antibody therapies against COVID-19.

• Description:

  The SARS-CoV-2 Spike Trimer (S1+S2) :ACE2 Inhibitor Screening Colorimetric Assay Kit is designed for screening and profiling inhibitors of the interaction of ACE2 (angiotensin-converting enzyme 2) with the Spike Trimer (S1+S2) protein. This kit comes in a convenient 96-well format, with purified SARS-CoV-2 Spike Trimer (S1+S2) (amino acids 16-1213) and ACE2-Biotin (amino acids 18-740) proteins, streptavidin-HRP, colorimetric HRP substrate, and assay buffer for 100 binding reactions. The SARS-CoV-2 Spike Trimer (S1+S2) protein included in the kit, provides a biologically relevant model for the investigation of SARS-CoV-2/ACE2 interaction.Figure 1. Assay Kit schematic.First, SARS-CoV-2 Spike Trimer (S1+S2) is coated on a 96-well plate overnight. After washing and blocking, the protein is pre-incubated with an inhibitor or neutralizing antibody. Upon subsequent incubation with Biotin-ACE2, the plate is treated with Streptavidin-HRP. After a final wash, Colorimetric HRP substrate is added to produce color that can be measured using a UV/Vis spectrophotometer microplate reader. The colorimetric signal is proportional to the efficacy of the binding of Spike Trimer (S1+S2) to ACE2.Need us to run an inhibitor screening or profiling? Check out ourCoronavirus Biochemical Screening Services.

• Applications:

  Screening inhibitors of ACE2 binding to SARS-CoV-2 Spike Trimer (S1+S2) .

• Format:

  CatalogNumberComponentAmountStorage100728Spike Trimer (S1 + S2), His-Tag (SARS-CoV-2) *5 µg-80°C100665ACE2, His-Avi-Tag, Biotin labeled*5 µg-80°C793113x Immuno Buffer 150 ml-20°C79728Blocking Buffer 250 ml+4°C79742Streptavidin-HRP10 µl+4°C79651Colorimetric HRP substrate10 ml+4°C79964Transparent 96-well microplate1RoomTemp*The initial concentration of the proteins is lot-specific and will be indicated on the tube containing the protein.

• Shipping Conditions:

  -80°C

• Storage Conditions:

  Up to 6 months from date of receipt, when stored as recommended.

• Contraindications:

  This assay kit is compatible with up to 1% final DMSO concentration.