TRKB Kinase Assay Kit

CAT: 0421-78591Size: 96 ReactionsDry Ice: YesHazardous: No

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CAT#:0421-78591Size:96 Reactions

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Background

Tropomyosin receptor kinase B (TRKB) is a high-affinity catalytic receptor for neurotrophins and is part of a large family of tyrosine kinases. Once activated by specific neurotrophins, such as brain derived neurotrophic factor (BDNF), TRKB mediates neuronal differentiation and survival. TRKB and BDNF have been associated with the pathology and progression of Alzheimer’s disease and other neurodegenerative disorders. More recently as a member of TRK proto-oncogene family, TRKB inhibitors have become of interest as a possible cancer therapy.

Description

The TRKB Kinase Assay Kit is designed to measure TRKB kinase activity for screening and profiling applications using ADP-Glo® as a detection reagent.

UniProt

Q16620

Applications

Study enzyme kinetics and screen small molecular inhibitors for drug discovery and high throughput (HTS) applications.

Format

Catalog #NameAmountStorage40281TRKB*10 µg-80°C79334Kinase Buffer 1 (5x) 1.5 ml-20°C79686ATP (500 µM) 50 µl-20°C40217PTK Substrate (Poly-Glu, Tyr 4:1) (10 mg/ml) 50 µl-20°CWhite 96-well plate1Room Temp*The concentration of the protein is lot-specific and will be indicated on the tube

Shipping Conditions

-80°C

Storage Conditions

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Notes

Troubleshooting GuideVisitbpsbioscience.com/assay-kits-faqfor detailed troubleshooting instructions. For all further questions, please email[email protected].

Contraindications

The final concentration of DMSO in the assay should not exceed 1%.

UniProtKB · Q16620

BDNF/NT-3 growth factors receptor

NTRK2_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: Q16620
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: NTRK2
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: —
EC number: 2.7.10.1
Processing: Precursor
Secondary accessions: B1ANZ4, B4DFV9, Q16675, Q59GJ1, Q8WXJ5, Q8WXJ6, Q8WXJ7

Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Ligand

ATP-bindingNucleotide-binding

Cellular component

Cell membraneCell projectionCytoplasmEndosomeMembraneSynapse

Molecular function

Developmental proteinKinaseReceptorTransferaseTyrosine-protein kinase

Biological process

DifferentiationNeurogenesis

Disease

Disease variantEpilepsyObesity

PTM

Disulfide bondGlycoproteinPhosphoproteinUbl conjugation

Domain

Immunoglobulin domainLeucine-rich repeatRepeatSignalTransmembraneTransmembrane helix

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Alternative Products

Catalog	Name
MBS2158413-01	Tropomyosin Receptor Kinase B (TrkB) Magnetic Fluorescence Assay Kit
LKU600448	Tropomyosin Receptor Kinase B (TrkB) Magnetic Luminex Assay Kit
TRV-057553	Multiplex Assay Kit for Tropomyosin Receptor Kinase B (TrkB), etc. by FLIA (Flow Luminescence Immunoassay)
NGO-IB27022-0384	Human TrkB Reporter Assay System
JL21132-01	Rat TrkB tyrosine kinase (TrkB) ELISA Kit
GTR18874539-01	Mouse TrkB tyrosine kinase (TrkB) ELISA Kit
171057	NTRK2 (Gp145-TrkB, TRKB, Trk-B, Tropomyosin-related Kinase B, TrkB Tyrosine Kinase, Tyrosine Kinase Receptor B) discontinued
T8663-21-01	TrkB (Tyrosine Kinase Receptor B)
AS13 2697	TrkB \| Tyrosine Kinase Receptor B
215490	TrkB-Fc Chimera, Human (Tropomyosin-Related Kinase Receptor, BDNF/NT-3 Growth Factors Receptor, Neurotrophic Tyrosine Kinase Receptor Type 2, TrkB Tyrosine Kinase, GP145-TrkB/GP95-TrkB, Trk-B, Ntrk2, Trkb, TrkB-Fc Chimera)